NACH OBEN

Publikationen

RNA-Thermometer

  • Guanzon DA, Pienkoß S, Brandenburg VB, Röder J, Scheller D, Dietze A, Wimbert A, Twittenhoff C, Narberhaus F:  Two temperature-responsive RNAs act in concert: The small RNA CyaR and the mRNA ompX. Nucleic Acids Res 53:gkaf04 (2025) PubMed
  • Scheller D, Becker F, Wimbert A, Meggers D, Pienkoß S, Twittenhoff C, Knoke LR, Leichert LI, Narberhaus F: The oxidative stress response, in particular the katY gene, is temperature-regulated in Yersinia pseudotuberculosis. PLoS Genet 19:e1010669 (2023) PubMed
  • Pienkoß S, Javadi S, Chaoprasid P, Holler M, Roßmanith J, Dersch P, Narberhaus F:  RNA thermometer-coordinated assembly of the Yersinia injectisome. J Mol Biol 34:167667. (2022) PubMed
  • Pienkoß S, Javadi S, Chaoprasid P, Nolte T, Twittenhoff C, Dersch P, Narberhaus F:  The gatekeeper of Yersinia type III secretion is under RNA thermometer control. PLoS Pathog 17:e1009650. (2021) PubMed
  • Brewer SM, Twittenhoff C, Kortmann J, Brubaker SW, Honeycutt J, Massis LM, Pham THM, Narberhaus F, Monack DM: A Salmonella Typhi RNA thermosensor regulates virulence factors and innate immune evasion in response to host temperature. PLoS Pathog 17:e1009345 (2021) PubMed
  • Twittenhoff C, Brandenburg VB, Righetti F, Nuss AM, Mosig A, Dersch P, Narberhaus F: Lead-seq: transcriptome-wide structure probing in vivo lead (II)ions. Nucleic Acids Res 48:e71 (2020) PubMed
  • Twittenhoff C, Heroven AK, Mühlen S, Dersch P, Narberhaus F: An RNA thermometer dictates production of a secreted bacterial toxin. PLoS Pathog 16:e1008184 (2020) PubMed
  • Loh E, Righetti F, Eichner H, Twittenhoff C, Narberhaus F: RNA thermometers in bacterial pathogens. Microbiol Spectr. 10.1128/microbiolspec.RWR-0012-2017  (2017) PubMed
  • Righetti F, Nuss AM, Twittenhoff C, Beele S, Urban K, Will S, Bernhart SH, Stadler PF, Dersch P, Narberhaus F: The temperature-responsive in vitro RNA structurome of Yersinia pseudotuberculosis. Proc Natl Acad Sci USA 113:7237-7242 (2016) PubMed
  • Roßmanith J, Narberhaus F: Exploring the modular nature of riboswitches and RNA thermometers. Nucleic Acids Res 44:5410-5423 (2016) PubMed
  • Weber GG, Kortmann J, Narberhaus F, Klose KE: An RNA thermometer controls temperature-dependent virulence factor expression in Vibrio cholerae. Proc Natl Acad Sci USA 111:14241-14246 (2014)
    Commentary in Science http://www.sciencemag.org/content/346/6207/twil.full.pdf (Film der Deutschen Welle) PubMed
  • Narberhaus F: RNAs at fever pitch. Nature (News & Views) 502:178-179 (2013) PubMed
  • Kortmann J, Narberhaus F: Bacterial RNA thermometers: Molecular zippers and switches. Nat. Rev. Microbiol. 10:255-265 (2012) PubMed
  • Böhme K, Steinmann R, Kortmann J, Seekircher S, Heroven AK, Berger E, Pisano F, Thiermann T, Wolf-Watz H, Narberhaus, F, Dersch P: Concerted actions of a thermo-labile regulator and a unique intergenic RNA thermosensor control Yersinia virulence. PLoS Pathog. 8:e1002518 (2012) PubMed
  • Kortmann J, Sczodrok S, Rinnenthal J, Schwalbe H, Narberhaus F: Translation on demand by a simple RNA-based thermosensor. Nucleic Acids Res. 39:2855-2868 (2011) PubMed
  • Waldminghaus T, Heidrich N, Brantl S, Narberhaus F: FourU - A novel type of RNA thermometer in Salmonella. Mol Microbiol 65:413-424 (2007) PubMed
  • Chowdhury S, Maris C, Allain FH, Narberhaus F: Molecular basis for temperature sensing by an RNA thermometer. EMBO J. 25:2487-97 (2006) PubMed

Genregulation in Agrobacterium tumefaciens

  • Schmidt JJ, Brandenburg VB, Elders H, Shahzad S, Schäkermann S, Fiedler R, Knoke LR, Pfänder Y, Dietze P, Bille H, Gärtner B, Albin LJ, Leichert LI, Bandow JE, Hofmann E, Narberhaus F: Two redox-responsive LysR-type transcription factors control the oxidative stress response of Agrobacterium tumefaciens. Nucleic Acids Res 53:gkaf267 (2025) PubMed
  • Remme DCLE, Tilg LJ, Pfänder Y, Yuan J, Narberhaus F: Small DUF1127 proteins regulate bacterial phosphate metabolism through protein-protein interactions with the sensor kinase PhoR. microLife 6:uqaf023 (2025) PubMed
  • Schmidt JJ, Remme DCLE, Eisfeld J, Brandenburg VB, Bille H, Narberhaus F: The LysR-type transcription factor LsrB regulates beta-lactam resistance in Agrobacterium tumefaciens. Mol Microbiol  doi: 10.1111/mmi.15191 (2024) PubMed
  • Eisfeld J, Kraus A, Ronge C, Jagst M, Brandenburg VB, Narberhaus F: A LysR-type transcriptional regulator controls the expression of numerous small RNAs in Agrobacterium tumefaciens. Mol Microbiol doi.org/10.1111/mmi.14695 (2021) PubMed
  • Kraus A, Weskamp M, Zierles J, Balzer M, Busch R, Eisfeld E, Lambertz J, Nowaczyk MM, Narberhaus F: Arginine-rich small proteins with a domain of unknown function DUF1127 play a role in phosphate and carbon metabolism of Agrobacterium tumefaciens. J Bacteriol 202:e00309-20 (2020) PubMed
  • Borgmann J, Schäkermann S, Bandow JE, Narberhaus F: A small regulatory RNA controls cell wall biosynthesis and antibiotic resistance. mBio 9:e02100-18 (2018) PubMed
  • Overlöper A, Kraus A, Gurski R, Wright PR, Georg J, Hess WR, Narberhaus F: Two separate modules of the conserved regulatory RNA AbcR1 address multiple target mRNAs in and outside of the translation initiation region. RNA biol. 11:624-640 (2014) PubMed
  • Wilms I, Overlöper A, Nowrousian M, Sharma CM, Narberhaus F: Deep sequencing uncovers numerous small RNAs on all four replicons of the plant pathogen Agrobacterium tumefaciens. RNA biol. 9:446-457 (2012) PubMed
  • Wilms I, Voss B, Hess W R, Leichert L I, Narberhaus F: Small RNA-mediated control of the Agrobacterium tumefaciens GABA binding protein. Mol Microbiol 80:492-506 (2011) PubMed

Regulierte Proteolyse und Lipopolysaccharid-Biosynthese

  • Dewachter L, Deckers B, Mares-Mejia I, Louwagie E, Vercauteren S, Matthay P, Brückner S, Möller AM, Narberhaus F, Vonesch SC, Versées W, Michiels J: The role of the essential GTPase ObgE in regulating lipopolysaccharide synthesis in Escherichia coli. Nat Commun 15:9684 (2020) PubMed
  • Möller AM, Vázquez-Hernández M, Kutscher B, Brysch R, Brückner S, Marino E, Kleetz J, Senges CHR, Schäkermann S, Bandow J, Narberhaus F: Common and varied molecular responses of Escherichia coli to five different inhibitors of the lipopolysaccharide biosynthetic enzyme LpxC. J Biol Chem 300:107143 (2024). PubMed
  • Brückner S, Müller F, Schadowski L, Kalle T, Weber S, Marino EC, Kutscher B, Möller AM, Adler S, Begerow D, Steinchen W, Bange G, Narberhaus F: (p)ppGpp and moonlighting RNases influence the first step of lipopolysaccharide biosynthesis in Escherichia coli. Microlife 4:uqad031 (2023) PubMed
  • Möller AM, Brückner S, Tilg LJ, Kutscher B, Nowaczyk MM, Narberhaus F: LapB (YciM) orchestrates protein-protein interactions at the interface of lipopolysaccharide and phospholipid biosynthesis. Mol Microbiol. 119:29-43. (2023) PubMed
  • Sauerbrei B, Arends J, Schünemann D, Narberhaus F: The Lon protease removes excess signal recognition particle protein in Escherichia coli. J Bacteriol 202:e00161-20 (2020) PubMed
  • Thomanek N, Arends J, Lindemann C, Barkovits K, Meyer HE, Marcus K, Narberhaus F: Intricate crosstalk between lipopolysaccharide, phospholipid and fatty acid metabolism in Escherichia coli modulates proteolysis of LpxC. Front Microbiol 9:3285 (2019) PubMed
  • Arends J, Griego M, Thomanek N, Lindemann C, Kutscher B, Meyer HE, Narberhaus F: An integrated proteomic approach uncovers novel substrates and functions of the Lon protease in Escherichia coli. Proteomics. 10.1002/pmic.201800080 (2018) PubMed
  • Bittner LM, Westphal K, Narberhaus F: Conditional proteolysis of the membrane protein YfgM by the FtsH protease depends on a novel N-terminal degron. J Biol Chem 290:19367-19378 (2015) PubMed
  • Schäkermann M, Langklotz S, Narberhaus F: FtsH-mediated coordination of lipopolysaccharide biosynthesis in Escherichia coli correlates with the growth rate and the alarmone (p)ppGpp. J Bacteriol 195:1912-1919 (2013) PubMed
  • Westphal K, Langklotz S, Thomanek N, Narberhaus F: A trapping approach reveals novel substrates and physiological functions of the essential protease FtsH in Escherichia coli. J Biol Chem 287:42962-42971 (2012) PubMed
  • Langklotz S, Baumann U, Narberhaus F: Structure and function of the bacterial AAA protease FtsH. BBA – Mol. Cell Res. 1823:40-48 (2012) PubMed
  • Langklotz S, Narberhaus F: The Escherichia coli replication inhibitor CspD is subject to growth-regulated degradation by the Lon protease. Mol Microbiol 80:1313-1325 (2011) PubMed
  • Langklotz S, Schäkermann M, Narberhaus F: Control of LPS biosynthesis by FtsH-mediated proteolysis of LpxC is conserved in enterobacteria but not in all Gram-negative bacteria. J Bacteriol 193:1090-1097 (2011) PubMed
  • Führer F, Langklotz S, Narberhaus F: The C-terminal end of LpxC is required for degradation by the FtsH protease. Mol Microbiol 59:1025-36 (2006) PubMed

Membran Biogenese

  • Kleetz J, Mizza AS, Shevyreva I, Welter L, Brocks C, Hemschemeier A, Aktas M, Narberhaus F: Three separate pathways in Rhizobium leguminosarum maintain phosphatidylcholine biosynthesis, which is required for symbiotic nitrogen fixation with clover. Appl Environ Microbiol 9:e0059024 (2024). PubMed
  • Vasilopoulos G, Heflik L, Czolkoss S, Heinrichs F, Kleetz J, Yesilyurt C, Tischler D, Westhoff P, Exterkate M, Aktas M, Narberhaus F: Characterization of multiple lysophosphatidic acid acyltransferases in the plant pathogen Xanthomonas campestris. FEBS J doi: 10.1111/febs.16996 (2024) PubMed
  • Czolkoss C, Borgert P, Poppenga T, Hölzl G, Aktas M, Narberhaus F: Synthesis of the unusual lipid bis(monoacylglycero)phosphate in environmental bacteria. Environ Microbiol 23:6993-7008. (2021) PubMed
  • Czolkoss S, Safronov X, Rexroth S, Knoke LR, Aktas M, Narberhaus F: Agrobacterium tumefaciens type IV and type VI secretion systems reside in detergent-resistant membranes. Front Microbiol 12:754486. (2021) PubMed
  • Kleetz J, Vasilopoulos G, Czolkoss S, Aktas M, Narberhaus F: Recombinant and endogenous ways to produce methylated phospholipids in Escherichia coli. Appl Microbiol Biotechnol 105:8837-8851. (2021) 
  • Kleetz J, Welter L, Mizza AS, Aktas M, Narberhaus F: Phospholipid N-methyltransferases produce various methylated phosphatidylethanolamine derivatives in thermophilic bacteria. Appl Environ Microbiol DOI: 10.1128/AEM.01105-21 (2021) PubMed
  • Vasilopoulos G, Moser R, Petersen J, Aktas M, Narberhaus F:  Promiscuous phospholipid biosynthesis enzymes in the plant pathogen Pseudomonas syringae. BBA Mol Cell Biol Lipids DOI: 10.1016/j.bbalip.2021.158926 (2021)
  • Knoke LR, Abad Herrera S, Götz K, Justesen BH, Günther Pomorski T, Fritz C, Schäkermann S, Bandow JE, Aktas M: Agrobacterium tumefaciens small lipoprotein Atu8019 is involved in selective outer membrane vesicle (OMV) docking to bacterial cells. Front Microbiol. 10.3389/fmicb.2020.01228 (2020) PubMed
  • Groenewold MK, Hebecker S, Fritz C, Czolkoss S, Wiesselmann M, Heinz DW, Jahn D, Narberhaus F, Aktas M, Moser J: Virulence of Agrobacterium tumefaciens requires lipid homeostasis mediated by the lysyl-phosphatidylglycerol hydrolase AcvB. Mol Microbiol 10.1111/mmi.14154 (2019) PubMed
  • Danne L, Aktas M, Unger A, Linke WA, Erdmann R, Narberhaus F: Membrane remodeling by a bacterial phospholipid-methylating enzyme. mBio 8:e02082-16 (2017) PubMed
  • Czolkoss S, Fritz C, Hölzl G, Aktas M: Two distinct cardiolipin synthases operate in Agrobacterium tumefaciens. PLoS One 11:e0160373 (2016) PubMed
  • Danne L, Aktas M, Gleichenhagen J, Grund N, Wagner D, Schwalbe H, Hoffknecht B, Metzler-Nolte N, Narberhaus F: Membrane-binding mechanism of a bacterial phospholipid N-methyltransferase. Mol Microbiol 95:313-331 (2015) PubMed
  • Moser R, Aktas M, Fritz C, Narberhaus F: Discovery of a bifunctional cardiolipin/ phosphatidylethanolamine synthase in bacteria. Mol Microbiol 92:959-972 (2014) PubMed
  • Aktas M, Danne L, Möller P, Narberhaus F: Membrane lipids in Agrobacterium tumefaciens: Biosynthetic pathways and importance for pathogenesis. Front Plant Sci. 5:109. doi: 10.3389/fpls.2014.00109 (2014) PubMed
  • Moser R, Aktas M, Narberhaus F: Phosphatidylcholine biosynthesis in Xanthomonas campestris via a yeast-like acylation pathway. Mol Microbiol 91:736-750 (2014) PubMed
  • Aktas M, Gleichenhagen J, Stoll R, Narberhaus F: S-adenosylmethionine (SAM)-binding properties of a bacterial phospholipid N-methyltransferase. J Bacteriol 193:3473-3481 (2011) PubMed
  • Aktas M, Narberhaus F: In vitro characterization of the enzyme properties of the phospholipid N-methyltransferase PmtA from Agrobacterium tumefaciens. J Bacteriol 191:2033-2041 (2009) PubMed
  • Wessel M, Klüsener S, Gödeke J, Fritz C, Hacker S und Narberhaus F: Virulence of Agrobacterium tumefaciens requires phosphatidylcholine in the bacterial membrane. Mol Microbiol 62: 906-915 (2006) PubMed

Bakterielles Prädationsverhalten

  • Kaimer C, Weltzer ML, Wall D: Two reasons to kill: predation and kin discrimination in myxobacteria. Microbiology 169:001372. (2023) PubMed
  • Thiery S, Turowski P, Berleman JE, Kaimer C: The predatory soil bacterium Myxococcus xanthus combines a Tad- and an atypical type 3-like protein secretion system to kill bacterial cells. Cell Rep 40:111340 (2022) PubMed
  • Thiery S, Kaimer C: The predation strategy of Myxococcus xanthus. Front. Microbiol. 11:2 (2020) doi: 10.3389/fmicb.2020.00002 PubMed
  • Thiery S & Kaimer C: Myxococcus xanthus - Einem bakteriellen Räuber auf der Spur. BioSpektrum 24: 449 (2020) doi: 10.1007/s12268-020-1390-6 
  • Arend K et al.: Myxococcus xanthus predation of Gram-positive or Gram-negative bacteria is mediated by different bacteriolytic mechanisms. Appl Env Microbiol (2020) doi: 10.1128/AEM.02382-20